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KMID : 0624620170500070384
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BMB Reports
2017 Volume.50 No. 7 p.384 ~ p.389
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Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis
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Jo Ha-Neul
Kang Hye-Soo
Lee A-Ram
Choi Ji-Hea
Chang Woo-Chul
Lee Myeong-Sok
Kim Jong-Min
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Abstract
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The Nogo-B receptor (NgBR) is necessary for not only Nogo-B-mediated angiogenesis but also vascular endothelial growth factor (VEGF)-induced angiogenesis. However, the molecular mechanisms underlying the regulatory role of the VEGF-NgBR axis in angiogenesis are not fully understood. Here, we report that miR-26a serves as a critical regulator of VEGF-mediated angiogenesis through directly targeting NgBR in endothelial cells (ECs). Stimulation of ECs by VEGF increased the expression of NgBR and decreased the expression of miR-26a. In addition, miR-26a decreased the VEGF-induced migration and proliferation of ECs. Moreover, miR-26a overexpression in ECs decreased the VEGF-induced phosphorylation of the endothelial nitric oxide synthase (eNOS) and the production of nitric oxide, which is important for angiogenesis. Overall, these data suggest that miR-26a plays a key role in VEGF-mediated angiogenesis through the modulation of eNOS activity, which is mediated by its ability to regulate NgBR expression by directly targeting the NgBR 3′-UTR.
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KEYWORD
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Angiogenesis, Endothelial nitric oxide synthase, Micro-RNA-26a, Nogo-B receptor, Vascular endothelial growth factor
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